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CyFlow™ CD103 APC

CyFlow™ CD103 APC
Application: Flow cytometry
Clonality: monoclonal
Target Species: Human, Non-Human Primates
Regulatory Status: RUO
Source Species: Mouse
Field of Interest: Immunophenotyping
Format/Fluorochrome: APC
Laser: Red
Excitation Maximum: 650 nm
Emission Maximum: 660 nm
Isotype: IgG1
Antigen: CD103
Alternative Name: HML-1, Integrin αE-subunit
Clone: Ber-ACT8
Order number: CR778480
HLDA Workshop HLDA V—WS Code A067 Quantity 100 tests Volume 1.0 mL Immunogen HTLV... more
Product information "CyFlow™ CD103 APC"
HLDA WorkshopHLDA V—WS Code A067
Quantity100 tests
Volume1.0 mL
ImmunogenHTLV 1-induced human T cell line MAPS16
Background InformationCD103 (Integrin αE) is an integrin subunit that is expressed on intraepithelial lymphocytes, epithelial dendritic cells, lamina propria-derived dendritic cells, a subpopulation of lamina propria T cells, a small subset of peripheral lymphocytes, namely T reg cells, and on activated and TGF-β stimulated lymphocytes. CD103 is in mature form cleaved into two disulfide-linked chains (C-terminal 150 kDa chain and N-terminal 25 kDa chain). It heterodimerizes with integrin β7 subunit to form αE/β7 integrin (mucosal lymphocyte 1 antigen), which through binding E-cadherin mediates homing of lymphocytes to the intestinal epithelium, and, in addition to the role in adhesion, may serve as an accessory molecule for intraepithelial lymphocyte activation.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Technical Data Sheet

Instruction for Use

ECDoC

Certificate Of Analysis

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* All prices are quoted net of the statutory value-added tax and shipping costs and possibly delivery charges, if not otherwise described

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